Viability of Basidiomycete fungal strains under different conservation methods : cryopreservation vs . freeze - drying processes
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چکیده
Resumen Cuatro hongos basidiomycetes, Agaricus blazei Murrill (Agaricomycetideae), Ganoderma lucidum (W.Curt.: Fr.) P. Karst. y Grifola frondosa (Dicks.: Fr.) S.F. Gray (Higher Basidiomycetes), y Pleurotus pulmonarius (Fr.) Quél. (Agaricomycetideae) fueron evaluados bajo tres métodos de conservación durante 12 meses, observando su viabilidad con el fin de establecer el mejor método de conservación. La cinética de crecimiento, producción de biomasa y polisacáridos fueron estudiados. Los métodos de conservación implementados incluyeron: agua destilada a 24 oC; aserrín y salvado de arroz con glicerol 10% a -20 oC; aserrín y salvado de arroz con glicerol 10% a -80 oC y; liofilización de biomasa con trehalosa o leche desnatada. Luego de realizar el análisis de los resultados de 12 meses de conservación, se determinó que el tratamiento de agua destilada a 24 oC fue el mejor método de conservación con el porcentaje de recuperabilidad más alto un 83,3% en el mes 12, seguido por el tratamiento de crioconservación a 80 oC donde se recuperó el 75%, sin afectar negativamente la producción de biomasa y polisacáridos. El tratamiento a -20 oC y la liofilización no fueron efectivos; con la crioconservación a -20 oC solo se recuperaron cepas en el primer mes y con la liofilización no fue posible recuperar cepas en el período de 12 meses evaluado.
منابع مشابه
Methods of Cryopreservation in Fungi
Traditional method of the routine subculturing by transfer of fungal cultures from staled to fresh media is not a very practical means of storing large numbers of fungal cultures. It is time-consuming, prone to contamination, and does not prevent genetic and physiological changes. At present, besides freeze-drying (lyophilization), cryopreservation seems to be the best preservation technique av...
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